Nrf2 Expression in Primary Murine Microglia Cells

March 03, 2020

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Abcam

Product Name:

Anti-Nrf2 antibody

Catalog Number:

ab89443

Clone Number:

Monoclonal

The purpose of the experiment was to detect changes in the expression levels of total Nrf2. Primary murine microglia cells were treated with 5µM of LPA for different time periods.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary murine microglia cells

Primary Incubation

1:1000, O/N, +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti mouse

Tertiary Incubation

N/A

Detection

ECL plus

Results Summary

No changes in the expression levels of Nrf2 were observed between treated and control samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works quite well

The Bad

N/A

The Bottom Line

A quite good antibody for western blot.

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