Abcam
Anti-CD68 antibody
ab125212
Polyclonal
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BV2 microglia cells were treated with 1µM LPA for different time periods. Changes in CD68 expression were detected using immunoblotting.
Western Blot
BV2 Microglia cells
1:500, O/N, +4
5% milk
1:5000 anti rabbit
N/A
ECL plus
Changes in CD68 expression were detected after 4hours treatment with LPA and the increased expression was observed at 8 and 24 hours as well.
May need exposure during detection for a long time
Works quite well
Quite good Ab for CD68 detection with western blotting.