GAPDH as a Loading Control

February 25, 2020

BlueskyReddit
Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-GAPDH antibody

Catalog Number:

ab9485

Clone Number:

Polyclonal

GAPDH Ab was used as a loading control for many WB experiments. The antibody is very good.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Microglia cells

Primary Incubation

1:1000, O/N

Blocking Agent

5% milk

Secondary Incubation

1:5000 anti rabbit

Tertiary Incubation

N/A

Detection

ECL

Results Summary

No changes in levels were detected (as expected).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works very well

The Bad

N/A

The Bottom Line

Very good antibody, used broadly as a loading control.

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