eNOS Expression in Disease Condition

SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
University of Nebraska
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

eNOS Antibody

Catalog Number:

eNOS (D9A5L) Rabbit mAb #32027

Clone Number:

N/A

Various diseases, such as atherosclerosis and diabetes, the function of endothelial nitric oxide (NO) synthase (eNOS) is altered, and that it produces superoxide instead of NO. This alteration is referred to as “eNOS uncoupling” and is linked to an increased monomerization of the enzyme. Here we performed disease condition in eNOS levels. We got nice expression of eNOS levels.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Tissue lysate and cell lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skimmed milk

Secondary Incubation

1:5000 dilution in 5% skim milk prepared in 1X TBST

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We found good result in disease condition.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background good signal

The Bad

None

The Bottom Line

This product is very strong signal. Highly recommended.

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