UCP2 Expression of Disease Condition

February 27, 2020

BlueskyReddit
SCHOOL OF VETERINARY MEDICINE AND BIOMEDICAL SCIENCES
University of Nebraska
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

UCP2 Antibody

Catalog Number:

89326

Clone Number:

N/A

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). It is separate oxidative phosphorylation from ATP synthesis and mainly involved in proton leak. Here we performed experiments in disease conditions; UCP2 levels are low.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Tissue lysate

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skim milk

Secondary Incubation

1:5000 dilution in 5% skim milk prepared in 1X TBST

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We performed UCP2 in disease condition and control. We got good results and good signal by using cell signaling UCP2 antibody.

DOI or PMID #

N/A

Additional Notes

Nil

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Summary

The Good

Good signal

The Bad

Nil

The Bottom Line

Uncoupling proteins (UCPs) are proton carriers, expressed in the mitochondrial inner membrane, that uncouple oxygen consumption by the respiratory chain from ATP synthesis. My experience with Cell Signaling UCP2 antibody is working good and we got good signal.

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