Good Antibody to Detect Elastin with Immunofluorescence

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Elastin Antibody

Catalog Number:

NB100-2076

Clone Number:

BA-4

The specific goal of this experiment was to evaluate extracellular matrix changes, in particular, elastin expression in the course of diseases. This antibody demonstrated excellent staining for elastin in feline tissues.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Feline connective tissues

Primary Incubation

Diluted at 1:50, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-mouse IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust fluorescence signals from the immunolabelling with this elastin antibody was detected in feline tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Highly specific with no background.

The Bad

Requires high concentration for immunofluorescence application.

The Bottom Line

Very specific elastin antibody for feline species.

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