Highly Specific Smooth Muscle Actin Antibody

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

Alpha-Smooth Muscle Actin Antibody

Catalog Number:

NBP1-30894

Clone Number:

N/A

Our research focuses on understanding multiple pathological processes in neurodegenerative diseases. The specific goal of this experiment was to evaluate the vascular morphology in the course of disease progression. This antibody demonstrated excellent staining for vessels in feline tissues.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Feline ocular tissues

Primary Incubation

Diluted at 1:400, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 488 conjugated anti-rabbit IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust immunofluorescence signals from the labelling for vessels was detected (green) in feline ocular tisseus.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very sensitive

The Bad

A little bit of background compared to monoclonal antibodies

The Bottom Line

A very specific vessel marker for feline species and generates bright signals (red).

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