Time Course Effect of MMP2 by Chemoattractant

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

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Quality of Results

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Company:

Santa Cruz Biotechnology

Product Name:

MMP2

Catalog Number:

SC-6838

Clone Number:

2C1

MMP-2 and TIMP-2 are present in the arterial wall of AAA as demonstrated by immunohistochemistry, in situ hybridization and in situ zymography. Aortic SMCs cultured from aneurysmal tissue express MMP-2 protein and mRNA at a significantly higher level than controls; a finding that was not present in other mesenchymal tissue.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell and mouse tissue lysate samples

Primary Incubation

1:1000 dilution in 5% skim milk prepared in 1X TBST

Blocking Agent

5% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found a time-dependent increase of MMP2 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and signal is strong. Highly recommended.

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