Cell Signaling Technology
eNOS
32027
Monoclonal
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NO is produced by the endothelium, it can regulate several aspects of vascular function via activation of the primary NO receptor, sGC, or initiate nitrosation reactions with iron-sulphur-centered proteins or proteins with reactive thiols (S-nitrosylation). here we checked time course effect of eNOS by chemoattractant.
Western Blot
Mouse tissue lysate samples
1:1000 dilution in 5% skim milk prepared in 1X TBST
5% skimmed milk
1:4000 5% skim milk prepared in 1X TBST
None
ECL
Our experimental results We found that time-dependent decrease of eNos by chemoattractant.
N/A
No background and very strong signal
This antibody is very specific and the signal is strong.