Western Blot for Catalase with Santa Cruz Antibody

Cellular/Molecular Biology
Texas Tech Health Sciences Center
Research Scientist

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Company:

Santa Cruz Biotechnology

Product Name:

Catalase

Catalog Number:

SC-271803

Clone Number:

H-9

Catalase is ubiquitously present in all aerobic cells (plants, animals, and microbes) containing a cytochrome system; only strict anaerobes seem to lack catalase activity. The enzyme is found in most cell tissues and cell organisms founded. In mammalian tissues there is considerable different, with catalase conc being high in liver cells and erythrocytes and low levels in connective tissues. Here we checked the time course effect of catalase by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue lysate samples

Primary Incubation

1:1000 dilution in 2% skim milk prepared in 1X TBST

Blocking Agent

2% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found a time-dependent decrease of catalase by chemoattractant

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody signal is strong and very specific.

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