Rabbit Anti-pSer149-CDC25B Review

January 28, 2020

BlueskyReddit
Biology
Nova Southeastern University
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Signalway

Product Name:

Rabbit anti-pSer149-CDC25B

Catalog Number:

11553

Clone Number:

Polyclonal

Good antibody with specificity for Western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse oocytes and eggs

Primary Incubation

1:200 in blocking buffer at 4 degrees C, overnight

Blocking Agent

5% non-fat milk in TBS, 0.1% Tween-20

Secondary Incubation

HRP-conjugated goat anti-rabbit (Genscript; 1 mg/ml stock) used at a dilution of 1:2000 in the blocking buffer for 2 hours at room temperature.

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

CDC25B seems phosphorylated at Ser-149 in mouse oocytes and but not in eggs.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Fairly strong signal with good specificity in Western blot.

The Bad

None

The Bottom Line

Great product for Western blot.

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