BioLegend
APC Streptavidin
405207
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This antibody works great, even when used at a fairly dilute (1:500) concentration. Easily and specifically binds biotin.
Flow Cytometry
iDCs polarized from isolated monocytes
30 minutes at 4*C with our titrated primary in-house biotinylated antibody
Fc blocked with Fcx Trustain 10' RT before adding primary antibody
30 minutes at 4*C with the Biolegend APC-Streptavidin antibody used at 1:500
No tertiary incubation
Flow cytometry
Detected our titration of biotinylated antibody beautifully.
N/A
Works great
No cons
This antibody worked very well, detecting our titrated biotinylated antibody when used at 1:500