Anti-Human CD56 ASR from BD Biosciences

Toxicology
University of Kentucky
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD56 PE

Catalog Number:

340685

Clone Number:

MY31

Browse Similar Products: CD56 Flow Cytometry Antibodies

This antibody works well. It is IgG1 which is a plus, especially when cocktailed. Titration of the antibody does indicate that the 20uL recommended vol/test is a good place to start.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human WBC prep

Primary Incubation

15 minutes; at 4C

Blocking Agent

None

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer (FL-2)

Results Summary

The antibody stained NK cells as expected. Our aim was to compare its performance to another CD56 clone (NCAM16.2 - see my other review). This antibody worked well, and its titration made sense going from 20uL as suggested by the manufacturer. Being an IgG1 is a bonus, especially when cocktailed with other antibodies.

DOI or PMID #

N/A

Additional Notes

Please see my NCAM16.2 review.

Image Gallery

Summary

The Good

Works well. Easy to use. IgG1 (and not IgG2)

The Bad

A little expensive.

The Bottom Line

This is a good antibody, and it works well once titrated optimally. It is good when used in a cocktail.

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