BUV395 Rat Anti-Mouse CD45 from BD Biosciences Works Great

January 31, 2020

BlueskyReddit
Innate Immunity
DRFZ
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV395 Rat Anti-Mouse CD45

Catalog Number:

564279

Clone Number:

30-F11

Browse Similar Products: CD45 Flow Cytometry Antibodies

The 30-F11 clone has been reported to react with all isoforms and both alloantigens of CD45, which is found on hematopoietic stem cells and all cells of hematopoietic origin, except erythrocytes. We used it to identify leukocyte progenitors in murine bone marrow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bone marrow cells

Primary Incubation

20mins 37oC

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

The antibody identified the CD45+ cells in murine bone marrow with high specificity.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good, sensitive, high resolution

The Bad

No bad things about it

The Bottom Line

I would recommend this antibody

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