Good CD28 Marker for Humans from BD Biosciences

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Bioscience

Product Name:

PE-CF594 Mouse Anti-Human CD28

Catalog Number:

562296

Clone Number:

CD28.2

Browse Similar Products: CD28 Flow Cytometry Antibodies

Our lab studies the function and make up of lymphocytes in various human tissues throughout life. In order to focus on function of these cells, we need a good and bright CD28 marker so we chose this one.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody shows us the positive CD28 signal on our lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This is a reliable antibody to stain CD28 expression on human lymphocytes.

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