Easy-to-Use Streptavidin from BioLegend

February 21, 2020

BlueskyReddit
Flowcore
University of Rochester
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Dazzle™ 594 Streptavidin

Catalog Number:

405248

Clone Number:

The goal is to detect a T cell population. The primary antibody was biotinylated.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat spleen

Primary Incubation

0.06ug per 100ul, 20 minutes on ice

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

A good distinctive and bright population stained with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good separation

The Bad

None

The Bottom Line

This is a good antibody to use as a secondary. It is bright.

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