Easy-to-Use CD335 Antibody from BioLegend

Flowcore
University of Rochester
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-rat CD335 (NKp46) Antibody

Catalog Number:

250806

Clone Number:

WEN23

The goal is to quantify NK cells in rat tissues. This antibody is easy to use but does not stain very brightly.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat spleen

Primary Incubation

0.25ug per 100ul, 20 minutes on ice

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

There is a very low frequency of cells that stained with this antibody. Cannot tell from this data whether the antibody is robust. Would need to try a different clone next time.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use

The Bad

Does not give a bright population

The Bottom Line

This antibody does not stain brightly. May need to try a different clone to see if results could be better.

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