Easy-to-Use CD11b/c Antibody from BioLegend

February 06, 2020

BlueskyReddit
Flowcore
University of Rochester
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-rat CD11b/c Antibody

Catalog Number:

201814

Clone Number:

OX-42

The purpose is to quantify non T cells in rat tissues. This antibody is easy to use but doesn't give a distinguishable population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat spleen

Primary Incubation

0.25ug per 100ul, 20 minutes on ice

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

There is no distinguishable population after staining with this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use

The Bad

Does not give a distinct population

The Bottom Line

This antibody is easy to use. However may need to use another antibody to help with forming a distinct population to gate on.

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