Easy-to-Use CD3 Antibody from BD Biosciences

February 03, 2020

BlueskyReddit
Flowcore
University of Rochester
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Biotin Mouse Anti-Rat CD3

Catalog Number:

554831

Clone Number:

G4.18

Browse Similar Products: CD3 Flow Cytometry Antibodies

The goal is to quantify T cells in spleen samples. The antibody is a good, reliable reagent.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Rat spleen

Primary Incubation

0.25ug per 100ul, 20 minutes on ice

Blocking Agent

Fc block

Secondary Incubation

Streptavidin

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Very good separation of positive and negative populations.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use

The Bad

None

The Bottom Line

Good, reliable reagent to detect T cells.

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