Excellent MAP2 Antibody from MAP2

February 03, 2020

cell biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

MAP2 Antibody

Catalog Number:

4542

Clone Number:

Polyclonal

I work on various aspects of neuronal cell biology. This antibody is useful as a neuronal marker

Experimental Design and Results Summary

Applications

Western Blot

Sample

H9 Human Embryonic Stem Cells and Induced Neuronal Cell Lysates

Primary Incubation

1:1000, Overnight, 4C

Blocking Agent

5% Milk TBST

Secondary Incubation

1:10,000, Room Temp, 1h

Tertiary Incubation

None

Detection

Enhanced Chemiluminescence using digital imager

Results Summary

Amazingly specific antibody with only minor background bands. Gave signal only in neuronal lysates indicating its utility in studying neuronal biology

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Detects all isoforms of Map2 and is clean

The Bad

Minor background bands

The Bottom Line

Must use if you need a good neuronal marker

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