A Good TRAIL Antibody from BioLegend

March 05, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE anti-mouse CD253 (TRAIL) Antibody

Catalog Number:

109305

Clone Number:

N2B2

I determined the expression of TRAIL on ectopically TRAIL expressing CT26 cell line. TRAIL belongs to TNF superfamily and induces apoptosis in cancer cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CT26 cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked well and I could detect the TRAIl+ cells in the cell populaiton.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background

The Bad

Nothing

The Bottom Line

Like most of the BioLegend antibodies, this antibody worked well for flow cytometry-based detection of TRAIL.

Share your experience with other scientists. Write a Review! »

Join the discussion