A Good CD140a Antibody from Thermo

March 04, 2020

BlueskyReddit
Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD140a (PDGFRA) Monoclonal Antibody (APA5), FITC, eBioscience™

Catalog Number:

11-1401-82

Clone Number:

APA5

I detected the expression of CD140 on CT26 cells. CD140a is a receptor tyrosine kinase which upon homo/heterodimerization activated downstream kinase activity.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CT26 cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked well and I could detect the expression of CD140a on CT 26 cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Ease of using it

The Bad

Non-specific background

The Bottom Line

This antibody works well but requires an extra wash to combat non-specific binding.

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