A Good IFN-Gamma Antibody from BioLegend

March 03, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse IFN-γ Antibody

Catalog Number:

505807

Clone Number:

XMG1.2

I tested the expression levels of intracellular IFN gamma in CD8+ T cells. This cytokine is a hallmark of activated CD8+ cytotoxic T cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked great and specifically binds to IFN gamma in T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

High specificity

The Bad

Nothing

The Bottom Line

This antibody performs well for flow cytometry-based detection of IFN gamma.

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