A Good TNF Alpha Antibody from BioLegend

March 02, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE anti-mouse TNF-α Antibody

Catalog Number:

506306

Clone Number:

MP6-XT22

I determined the intracellular levels of TNF alpha in CD8+ T cells isolated from murine spleen. It is one of the activation markers of T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody performed well for the detection of TNF alpha in T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No non-specific staining

The Bad

Nothing

The Bottom Line

It is a highly specific antibody that can be used for the detection of TNF alpha by flow cytometry.

Share your experience with other scientists. Write a Review! »

Join the discussion