A Good IL-2 Antibody from BioLegend

February 28, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse IL-2 Antibody

Catalog Number:

503808

Clone Number:

JES6-5H4

I tested the expression of intracellular IL-2 in CD8+ T cells upon stimulation with the antigen of our interest. IL-2. It is a cytokine that helps in the expansion of T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CD8+ T cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

A minor percentage of T cell expressed IL-2 upon stimulation with the antigen.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background

The Bad

Nothing

The Bottom Line

Overall, it is a great antibody for flow cytometry-based assays.

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