A Good CD24 Antibody from BioLegend

February 27, 2020

BlueskyReddit
Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

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Quality of Results

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Company:

BioLegend

Product Name:

Pacific Blue™ anti-mouse CD24 Antibody

Catalog Number:

101819

Clone Number:

M1/69

I culture murine bone marrow cells in the presence of GM-CSF for a week and characterized non-adherent cells. CD 24 is one of the dendritic cell markers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow derived dendritic cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

Most of the non-adherent cells expressed CD24 marker.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

High specificity

The Bad

Nothing

The Bottom Line

It is a good antibody for the detection of CD24 on dendritic cells by flow cytometry.

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