A Good CD11c Antibody from BioLegend

February 26, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

APC anti-mouse CD11c Antibody

Catalog Number:

117310

Clone Number:

N418

I used this antibody to characterize dendritic cells isolated from murine-derived bone cells in the presence of GM-CSF. CD11c is a membrane glycoprotein that helps in cell-cell contact.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow derived dendritic cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked well for the detection of CD11c on dendritic cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High specificity

The Bad

Nothing

The Bottom Line

This antibody can be successfully used to detect CD11c by flow cytometry.

Share your experience with other scientists. Write a Review! »

Join the discussion