A Good CD11b Antibody from BioLegend

February 25, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse/human CD11b Antibody

Catalog Number:

101211

Clone Number:

M1/70

I cultured murine bone marrow cells in the presence of GM-CSF to isolate dendritic cells. I used this antibody to characterize dendritic cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow derived dendritic cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

Most of the cells isolated were positive for CD11b marker.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

It is a good antibody for flow cytometry-based detection of CD11b.

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