A Good CD80 Antibody from BioLegend

February 24, 2020

BlueskyReddit
Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

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Company:

BioLegend

Product Name:

FITC anti-mouse CD80 Antibody

Catalog Number:

104705

Clone Number:

16-10A1

I stained mouse Bone marrow derived denritic cells using his antibody. It is one of the maturation markers used for dendritic cell characterization.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse bone marrow derived dendritic cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody probed CD80 on dendritic cells. I collected non-adherent cells and characterized them

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

It is a good antibody for dendritic cell characterization.

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