A Good Ki67 Antibody from BioLegend

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse Ki-67 Antibody

Catalog Number:

652409

Clone Number:

16A8

I stained mouse splenocytes to probe for Ki67 on activated T cells. Ki67 is a proliferation marker and I did intracellular staining to probe for it.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody specifically binds to Ki67 and I was able to probe Ki67 expressing T cells in mouse splenocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High specificity

The Bad

Nothing

The Bottom Line

Overall, it is a good antibody to detect Ki67 by Flow cytometry

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