A Good CD58 Antibody from Thermo

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD58 Monoclonal Antibody (MEM-63), FITC

Catalog Number:

MA1-19575

Clone Number:

MEM-63

I detected the expression of CD 58 on T cells present in human PBMCs. It is a cell adhesion transmembrane molecule present on antigen presenting cells and lymphocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human Peripheral Blood mononuclear cells.

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody could successfully recognize cells expressing CD58 marker in human PBMCs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

It's a great antibody for flow cytometry-based assays.

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