A Good CD9 Antibody from R&D Systems

February 14, 2020

BlueskyReddit
Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Mouse CD9 Antibody

Catalog Number:

MAB5218

Clone Number:

479608

I stained mouse CT26 cells with this antibody to detect the expression of CD9. CD9 is known to be associated with cell adhesion, migration, and invasion.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CT26 cell line

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody recognizes and specifically binds to the protein CD9 present in CT26 cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background

The Bad

I got the weak intensity which could be perhaps low expression of CD9 on CT26 cells.

The Bottom Line

The antibody works great for flow cytometry-based detection of CD9.

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