Good Anti-CD326 Antibody from Thermo

February 13, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD326 (EpCAM) Monoclonal Antibody (1B7), Super Bright 702, eBioscience™

Catalog Number:

67-9326-42

Clone Number:

1B7

I transfected HEK 293 T cells with EpCAM expressing lentivirus and monitored the transduction efficacy by using this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

HEK 293 T cells

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked really well in detecting EpCAM expressing cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background

The Bad

Nothing

The Bottom Line

This antibody is useful for flow cytometry based detection of EpCAM.

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