Good CD282/TLR2 Antibody from BioLegend

February 11, 2020

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD282 (TLR2) Antibody

Catalog Number:

148603

Clone Number:

CB225

I stimulated THP-1 cells (human monocytes) with 100 micromolar phorbol 12-myristate 13-acetate (PMA) for 2 hours and determined the expression of TLR2 on these cells using this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP-1

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

Activation of TLR2 was detected on THP-1 cells post-stimulation. This antibody served the purpose of TLR2 detection.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

Overall, it is a good antibody

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