Good CD271 Antibody from BioLegend

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human CD271 (NGFR) Antibody

Catalog Number:

345105

Clone Number:

ME20.4

It is a membrane protein present in different mammalian cells. I determined its expression on REH cell line (origin: Acute Lymphocytic Leukemia).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

REH cell line

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

This antibody worked well for the detection of CD271 in this cell line.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background

The Bad

Nothing

The Bottom Line

It's a good antibody for flow cytometry

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