A Good CD146 Antibody from R&D Systems

Medicine
Brigham and Women's Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Rat MCAM/CD146 PE-conjugated Antibody

Catalog Number:

FAB3250P-100UG

Clone Number:

404722

I detected the expression of CD 146 on TPC-1 (thyroid cancer cell line). Melanoma Cell Adhesion Molecule or CD146 is a cell adhesion protein that is expressed on endothelial cells, neurons and tumor cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

TPC-1 cell line

Primary Incubation

1:100 at 4 degrees for 30 minutes

Blocking Agent

2% FBS in PBS

Secondary Incubation

Not applicable

Tertiary Incubation

Not applicable

Detection

Flow cytometry

Results Summary

The data was clear and specific. Antibody worked well for this cell line.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High specificity

The Bad

Nothing

The Bottom Line

It's a great antibody for flow cytometry-based detection of CD146

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