Good CD57 Antibody from Miltenyi Biotec

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti CD57 Antibody

Catalog Number:

130-092-174

Clone Number:

TB03

The focus of this project was to investigate by a multiplex immunfluorescence technique the involvement of certain proteins in the pathogenesis of the Alzheimer's disease . One of our markers of interest was CD57.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human brain (choroid plexus)

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signal with some background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody labelled the cells of interest.

The Bad

Degree of labeling (DOI) for this antibody is improvable.

The Bottom Line

This antibody works

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