Good CD27 Antibody from Miltenyi Biotec

February 26, 2020

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti CD27 Antibody

Catalog Number:

130-113-629

Clone Number:

M-T271

In this project the different phenotypes of immune cells in human skin and lymph node were investigated by a multiplex immunfluorescence technique. CD27 was one of many marker in our analysis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human lymph node

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signals on frozen-tissue sections with some background. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody works

The Bad

Took some time to establish

The Bottom Line

Will buy and use it again

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