Easy-to-Use and Sensitive Antibody for O-Linked N-Acetylglucosamine

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-O-Linked N-Acetylglucosamine antibody

Catalog Number:

ab2739

Clone Number:

RL2

The antibody detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-Linked glycoproteins. We wish to see the changes in the level of glycosylation upon treatment with different types of stress.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine myeloid cells

Primary Incubation

1:1000, O/N, 4 degree C

Blocking Agent

3% BSA

Secondary Incubation

1:10000, 1hr, RTP

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

There are multiple bands seen and this is expected as many proteins can be glycosylated. We can see the differences in the global glycosylation patterns.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use and sensitive

The Bad

NIL

The Bottom Line

A easy to use and sensitive antibody. It is highly recommended to those who need it.

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