Excellent CD45 Antibody from Miltenyi Biotec

February 25, 2020

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti CD45 Antibody

Catalog Number:

130-113-117

Clone Number:

5B1

Our lab was interested in the charaterization of immune cells subtypes in inflammed skin diseases like psoriasis, pyoderma gangrenosum and ulcus cruris.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signal on frozen-tissue sections with little background. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Also a great antibody for IF (not only FC)

The Bad

None

The Bottom Line

Will buy and use it again. Recommendable!

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