Excellent CD44 Antibody from Miltenyi Biotec

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti CD44 Antibody

Catalog Number:

130-113-334

Clone Number:

DB105

In this project the pathogensis of melanoma was investigated. With the MELC technology, we analyzed protein expression profiles in nevi and melanomas. Here we focused on the CD44 expression.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (melanoma)

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives very specific and strong signals on frozen-tissue sections with no background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Also a great antibody for IF (not only FC)

The Bad

None

The Bottom Line

Will buy and use it again. Recommendable!

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