Very Good CD45RO Antibody from Miltenyi Biotec

January 30, 2020

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti CD45RO Antibody

Catalog Number:

130-113-549

Clone Number:

UCHL1

Our lab was interested in the characterization of immune cells subtypes in inflamed skin diseases like psoriasis, pyoderma gangrenosum, and ulcus cruris. This antibody gives a specific signal.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signal on frozen-tissue sections with little background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody works

The Bad

None

The Bottom Line

Also a great antibody for IF (not only FC)

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