Excellent CD45RA Antibody from Miltenyi Biotec

January 21, 2020

BlueskyReddit
Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti CD45RA Antibody

Catalog Number:

130-113-355

Clone Number:

T6D11

Our lab was interested in the characterization of immune cell subtypes in inflamed skin diseases like psoriasis, pyoderma gangrenosum, and ulcus cruris. This antibody gives specific and strong signals.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin

Primary Incubation

1:40; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives very specific and strong signals on frozen-tissue sections with no background. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Also a great antibody for IF (not only FC)

The Bad

None

The Bottom Line

Will buy and use it again. Recommendable!

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