Good CD25 Antibody from BioLegend

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD25 Antibody

Catalog Number:

302616

Clone Number:

BC96

In this project the pathogenesis of melanoma was investigated. With the MELC technology, we analyzed protein expression profiles in nevi and melanomas. Here we focused on the CD25 expression in Lymphocytes.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (melanoma)

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (AF488) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific signals on frozen-tissue sections with some background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works quite well

The Bad

Some Background

The Bottom Line

Recommendable!

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