Great CD45.1 Antibody from BioLegend

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD45.1 Antibody

Catalog Number:

110708

Clone Number:

A20

In this project the different phenotypes of immune cells in murine skin and lymph node were investigated by a multiplex immunfluorescence technique. These immune cells were attracted and activated by injected extracellular vesicles from mature murine dendritic cells. CD45.1 was one of many marker in our analysis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine lymph node

Primary Incubation

1:50; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (PE) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives specific and strong signals on frozen-tissue sections with no background. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody gives specific signal on frozen-tissue sections.

The Bad

None

The Bottom Line

Recommendable!

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