Good B220 BV711 from BioLegend

February 04, 2020

BlueskyReddit
Charite Strasse 1
Deutsches Rheuma-Forschungszentrum
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse/human CD45R/B220 Antibody

Catalog Number:

103255

Clone Number:

RA3-6B2

B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to identify B cells with B220 and CD19.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Wild-type mouse spleen

Primary Incubation

Surface staining with, B220 BV711 (1/400) CD19, etc (20 min, 4° in the dark)

Blocking Agent

Clone 2.4G2 (Fc block)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry, violet laser

Results Summary

I could stain B cells (B220+CD19+) in the spleen.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody works well

The Bad

None

The Bottom Line

Excellent option

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