Great USP22 Antibody from Abcam

January 22, 2020

BlueskyReddit
LMP
University of Toronto
Graduate Student

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Company:

Abcam

Product Name:

USP22 antibody

Catalog Number:

ab195289

Clone Number:

EPR18945

USP22 is member of the SAGA DUBm complex that is responsible for the acetylation and deubiquitination of chromatin. Here we assess the expression of USP22 in acute myeloid leukemia cell line OCI AML-2.

Experimental Design and Results Summary

Applications

Western Blot

Sample

AML2 whole cell lysate

Primary Incubation

1:1000 RT 1hr in 5% skim milk

Blocking Agent

5% milk - 10 min room temperature

Secondary Incubation

5% milk - 30 min room temperature secondary Ab 1:10K

Tertiary Incubation

N/A

Detection

ECL - film

Results Summary

Good antibody for western blot with low background. Approx. size 60 kDa. Conditions represent samples following CRISPR/Cas9 deletion of USP22 using two different sgRNAs. sgLacZ represents non-targeting control. GAPDH is shown as the loading control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good USP22 Antibody.

The Bad

Nothing

The Bottom Line

Clean USP22 antibody for detection in human cell lysates.

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