Good ENY2 Antibody from GeneTex

January 21, 2020

LMP
University of Toronto
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

ENY2 antibody

Catalog Number:

GTX128034

Clone Number:

Polyclonal

ENY2 is member of the SAGA DUBm complex that is responsible for the acetylation and deubiquitination of chromatin. Here we assess the expression of ENY2 in acute myeloid leukemia cell line OCI AML-2.

Experimental Design and Results Summary

Applications

Western Blot

Sample

AML2 whole cell lysate

Primary Incubation

1:1000 RT 1hr

Blocking Agent

5% skimmed milk - 10 min room temperature

Secondary Incubation

5% skimmed milk - 30 min room temperature Rb secondary Ab 1:10K

Tertiary Incubation

N/A

Detection

ECL reagent – film

Results Summary

Good antibody for western blot with low background. Approx. size 12 kDa. Conditions represent samples following CRISPR/Cas9 deletion of ENY2 using two different sgRNAs. sgLacZ represents non-targeting control. GAPDH is shown as the loading control.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well.

The Bad

Company not well known, so difficult to find distributor for ordering.

The Bottom Line

Good Antibody that detects ENY2 in human cell lysates.

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