Apoptosis and Survival Protein Signaling Array

This apoptosis kit was very user-friendly and gave detailed instructions on how to analyze findings using a software like Excel. This assay was very helpful for examining multiple gene targets simultaneously, so we got a lot of data out of 1 experiment. Negative and positive controls were included on the blots, which was useful in subsequent analyses. This assay gives you a lot of data regarding the levels of important proteins involved in cell survival and cell death signaling pathways. For interpretation of results, it would be helpful if the manufacturer denoted which proteins specifically are involved in apoptosis and which may be associated with inhibiting apoptosis. Otherwise, I found this to be a very easy to use kit with clear instructions and I would recommend this for discovery purposes. Notes: the Caspase 3 antibody seemed a lot more sensitive than other antibodies on the blot. Also, some of the gene names in the kit manual differed from those listed on the website, so keep that in mind when analyzing data to ensure that the correct gene is being referred to.