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Brilliant Violet 510™ anti-mouse CD3ε Antibody
100353
145-2C11
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B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to counterstain T cells.
Flow Cytometry
Wild-type mouse spleen
Surface staining with, CD3 BV510 (1/100) CD19, etc (20 min, 4° in the dark)
Clone 2.4G2 (Fc block)
N/A
Flow cytometry, violet laser
I could stain CD3e+ T cells in the spleen.
The antibody works
The staining is very weak
It can be used